Akali C18

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When developing methods for peptides and basic compounds, you often can't avoid issues like pH selectivity, alkaline cleaning and regeneration, and scale-up stability. Whether the filler has a wider pH tolerance, higher mechanical strength, and consistent batch performance directly affects the efficiency of method development and long-term costs.

Akali C18 is a hybrid C18 packing material with 10 µm particles and 120 Å pores, designed for peptide prep and purification across a wide pH range. It maintains good column efficiency and longevity from pH 1.5 to 12, has uniform particle and pore size distribution, and a smooth surface, giving it excellent mechanical strength, separation performance, and batch-to-batch consistency.

Wide pH applicability allows better use of different pH selectivity for separation and purification.
Surface ethyl hybrid technology ensures longer lifespan.
Especially suitable for the analysis and purification of basic compounds.
Special stationary phase selectivity design is more favorable for the separation and purification of peptide compounds.

Parameter	Akali C18
固定相	C18
粒径 / 孔径	10 µm / 120 Å
pH 耐受范围	1.5–12.0
表面积	300–370 m²/g
碳载量	16.2%
最大耐受压力	30 MPa
最大耐受温度	60℃*

Tolerance: Open up the method space

A wider pH range means you can be more flexible in controlling the ionization state of the sample, retention behavior, and impurity separation when developing methods. In alkaline aging tests, Akali C18 remained stable in column efficiency even after 260 hours under conditions of methanol/25 mM sodium phosphate aqueous solution at pH 11.5 = 1/5.

In pure water tolerance tests, using 100% water as the mobile phase, the column efficiency also remained stable after 260 hours.

Mechanical strength: Focused on long-term usability for fabrication

When making the filler, it not only needs to 'separate well', but also withstand filling, operation, regeneration, and repeated use.

Under stress tolerance testing

Under methanol/water = 1/1 conditions, when the pressure of Akali C18 was increased from 20 MPa to 30 MPa, the column efficiency and tailing factor remained stable even after testing for 302 hours.

After repeatedly reloading the DAC

Akali C18 still maintains good column efficiency, and no particle breakage was seen under the electron microscope.