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CAPCELL PAK SEC Series

Product Details Ordering Information Download Materials Related video Application Cases

CAPCELL PAK SEC Molecular Sieving Mode Column

The CAPCELL PAK SEC series of columns is specially developed for analyzing compounds like peptides, proteins, and nucleic acids. With its excellent performance and wide range of applications, it provides strong support for researchers and analysts in fields such as biomedicine, food safety, and environmental monitoring.

Product features:

Silica-Based Size Exclusion Column:

Made with high-quality silica as the base, it offers excellent stability, ensuring that CAPCELL PAK SEC can be used reliably over the long term. Plus, the size exclusion mode makes this product especially suitable for analyzing large molecules like peptides, proteins, and nucleic acids.

Exclusive technology offers better separation performance:

Using a unique production technique, it ensures excellent separation, effectively isolating target molecules from impurities and improving analysis accuracy.

Lower Batch-to-Batch Variability:

As a product from a professional silica gel manufacturer, the CAPCELL PAK SEC series columns are designed with lower batch-to-batch differences in mind right from the silica selection stage. This means that columns purchased at different times by users will have highly consistent performance, ensuring reproducibility and reliability of experimental results.

Pore Size Options for Various Molecular Weight Ranges:

Different pore sizes can handle different molecular weight ranges, making the CAPCELL PAK SEC series columns widely applicable in various analytical applications.

CAPCELL PAK SEC Physical Parameters Table for Columns of Various Specifications

Column type	Aperture （Å）	Particle size （μm）	Scope division	pH range	Withstand pressure （Mpa）
CAPCELL PAK SEC	125	2	5 - 150k Da (Protein)	2-7.5	35/25
CAPCELL PAK SEC	125	5	5 - 150k Da (Protein)	2-7.5	7
CAPCELL PAK SEC	125	10	5 - 150k Da (Protein)	2-7.5	2.5
CAPCELL PAK SEC	250	2	10 - 500k Da (Protein)	2-7.5	35/25
CAPCELL PAK SEC	250	5	10 - 500k Da (Protein)	2-7.5	7
CAPCELL PAK SEC	250	10	10 - 500k Da (Protein)	2-7.5	2.5
CAPCELL PAK SEC	1000	5	100 - 7500k Da (Protein) 30 - 3000 nt(ssRNA)	2-7.5	7

CAPCELL PAK SEC125 Calibration Curve

【Liquid phase conditions】

Column：7.8 mm i.d. × 300 mm

Mobile Phase：100 mmol/L Phosphate buffer + 300 mmol/L NaCl (pH 7.0)

Flow rate：1.0 mL/min

Temperrature：25 ℃

Detector：UV 220 nm

Injection volumn：20 µL

Sample：1、670,000 Da，2、156,000 Da，3、75,000 Da，4、43,000 Da，5、13,700 Da，6、6,500 Da，7、246 Da

【Liquid phase conditions】

Column：4.6 mm i.d. × 300 mm

Mobile Phase：100 mmol/L Phosphate buffer + 300 mmol/L NaCl (pH 7.0)

Flow rate：0.35 mL/min

Temperrature：25 ℃

Detector：UV 220 nm

Injection volumn：10 µL

Sample ：1、670,000 Da，2、156,000 Da，3、75,000 Da，4、43,000 Da，5、13,700 Da，6、6,500 Da，7、246 Da

CAPCELL PAK SEC250 Calibration Curve

【Liquid phase conditions】

Column：7.8 mm i.d. × 300 mm

Mobile Phase：100 mmol/L Phosphate buffer + 300 mmol/L NaCl (pH 7.0)

Flow rate：1.0 mL/min

Temperature：25 ℃

Detector：UV 220 nm

Injection volumn：20 µL

Sample：1、1,340,000 Da，2、670,000 Da，3、156,000 Da，4、75,000 Da，5、43,000 Da，6、13,700 Da，7、6,500 Da，8、246 Da

【Liquid phase conditions】

Column：4.6 mm i.d. × 300 mm

Mobile phase：100 mmol/L Phosphate buffer + 300 mmol/L NaCl (pH 7.0)

Flow rate：0.35 mL/min

Temperature：25 ℃

Detector：UV 220 nm

Injection volumn：10 µL

Sample：1、1,340,000 Da，2、670,000 Da，3、156,000 Da，4、75,000 Da，5、43,000 Da，6、13,700 Da，7、6,500 Da，8、246 Da

CAPCELL PAK SEC1000 Calibration Curve

【Liquid phase conditions】

Column：7.8 mm i.d. × 300 mm

Mobile phase：20 mmol/L Phosphate buffer

Flow rate：0.3 mL/min

Temperature：60 ℃

Detector：UV 260 nm

Sample：1 nt - 9,000 nt

Standard protein analysis example

【Liquid phase conditions】

Column：CAPCELL PAK SEC125 S5 7.8 mm i.d. x 300 mm

Mobile phase：100 mmol/L Phosphate buffer + 100 mmol/L Na2SO4 (pH 6.7) + 0.05% NaN3

Temperature：25 ℃

Flow rate：1.0 mL/min
Detector：UV 280 nm

Injection volumn：20 µL

Sample：Proteins，1、Thyroglobulin (670,000 Da)，2、Ovalbumin (43,000 Da)，3、Ribonuclease A (13,700 Da)，4、p-Aminobenzoic acid (137 Da)

【Liquid phase conditions】

Column：CAPCELL PAK SEC250 S5 7.8 mm i.d. x 300 mm

Mobile phase：100 mmol/L Phosphate buffer + 100 mmol/L Na2SO4 (pH 6.7) + 0.05% NaN3

Temperature：25 ℃

Flow rate：1.0 mL/min
Detector：UV 280 nm

Injection volumn：20 µL

Sample：Proteins，1’、Thyroglobulin dimer (1,340,000 Da)，1、Thyroglobulin (670,000 Da)，2、Ovalbumin (43,000 Da)，3、Ribonuclease A (13,700 Da)，4、p-Aminobenzoic acid (137 Da)

【Liquid phase conditions】

Column：CAPCELL PAK SEC250 S2 4.6 mm i.d. x 300 mm

Mobile phase：100 mmol/L Phosphate buffer + 100 mmol/L Na2SO4 (pH 6.7) + 0.05% NaN3

Temperature：25 ℃

Flow rate：0.35 mL/min
Detector：UV 280 nm

Injection volumn：10 µL

Sample：Proteins，1、Thyroglobulin (670,000 Da)，2、γ-Globulin dimer(312,000 Da)，3、γ-Globulin(156,000 Da)4、Ovalbumin (43,000 Da)，5、Ribonuclease A (13,700 Da)，6、p-Aminobenzoic acid (137 Da)