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Product Category
HanKing PurBridge Series
HanKing PurBridge | Wide pH Alkali-resistant Type
HanKing PurBridge C18
Stationary phase | C18 |
Particle size（μm） | 1.8；3；5 |
Apertur（Å） | 150 |
Specific surface area（m²/g） | 190 |
Carbon load（C%） | 16% |
Seal end | 是 |
pH range | 1–12 |
USP | L1 |
PurBridge C18 uses fully hybrid particle technology and surface deactivation treatment to minimize the activity of silanol groups that may affect the peak shapes of basic compounds. It has a wide pH tolerance range of 1-12, with excellent durability and stability, making it very suitable for the separation of chelating compounds and basic compounds. It can withstand 100% aqueous phases, making it an ideal choice for method development under wide pH conditions.
Features
- Organic hybrid silica gel particles
- Double end-capped
- Extremely high durability and stability
- Very suitable for the separation of coordination compounds and basic compounds
- Can withstand 100% aqueous phase
Application Cases
Analysis of related substances of berberine hydrochloride
Column Condition
Column： | PurBridge C18，5 μm 250 x 4.6 mm |
Mobile Phase ： | 10 mM ammonium dihydrogen phosphate solution (pH adjusted to 2.8 with phosphoric acid) / acetonitrile (77:23) |
Flow rate： | 1.0 ml/min |
Column Temperature： | 15℃ |
Dectator： | UV@345 nm |
Injection volume： | 10 μL |
Bitter almond (reproduced by pharmacopoeia method)
Column Condition
Column： | PurBridge C18，5 μm 250 x 4.6 mm |
Mobile Phase ： | 0.1% Phosphoric Acid / Acetonitrile（92∶8） |
Flow rate： | 1.0 ml/min |
Column Temperature： | 25℃ |
Dectator： | UV@207 nm |
Injection volume： | 10 μL |
Separation of Degradation Impurities of Sacubitril Hydrochloride
Column Condition
Column： | PurBridge C18，3 μm，250 x 4.6 mm |
Mobile Phase： | A：50mM K2HPO4 B：ACN |
Flow rate： | 1.0ml/min |
Column Temperature： | 30℃ |
Dectator： | UV@220 nm |
Injection volume： | 10 μL |
Sample: | Sacubitril hydrochloride ester (2 mg/mL) Hydrolysis product (15 μg/mL) Photolysis products 1/2/3 (2 μg/mL) |
Elution Gradient
Time（min | A(%) | B(%) |
0.0 | 75 | 25 |
5.0 | 75 | 25 |
6.0 | 70 | 30 |
36.0 | 65 | 35 |
42.0 | 65 | 35 |
42.1 | 75 | 25 |
Determination of Benzalkonium Bromide Content
Column Condition
Column： | PurBridge C18，5 μm 250 x 4.6 mm |
Mobile Phase： | 5 mM ammonium acetate solution (containing 10 ml triethylamine per 1000 ml, pH adjusted to 5.0±0.5 with glacial acetic acid) / acetonitrile (25/75) |
Flow rate： | 1.5ml/min |
Column Temperature： | 35℃ |
Dectator： | UV@214 nm |
Injection volume： | 20 μL |
Sample: | Benzalkonium bromide preparation |
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Address: Building 2, 8th Floor, No. 248 Guanghua Road, Minhang District, Shanghai市
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