Applikon BioSep

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Scalable Cell Culture Perfusion Device

Applikon BioSep It is a specially designed cell-retention device used for continuously adding fresh culture medium and removing spent medium while retaining a large number of viable cells. The device employs high-frequency resonant ultrasound to separate cells, thereby automatically removing cellular debris, making it an outstanding solution for continuous bioprocessing.

Overview

The ideal solution for long-term cultivation

Automatically removes cell debris
Will not damage cells
Free of dirt, non-clogging, ensuring long-term operation.
Expandable perfusion device 0.1 – 1,000 rise / Heaven)

Features

High separation efficiency

Using acoustic resonance technology, high separation efficiency can be achieved without damaging any cells. (99%) For example, one standard operating mode involves installing a collection pump at the outlet of the resonant cavity and a corresponding circulation pump for the separated cells that have settled out from the acoustic energy field inside the resonant cavity, or alternatively, setting up... Applikon BioSep The system serves as an alternative strategy for achieving semi-continuous operation or cell recycling.

Easy to install and operate

Applikon BioSep It can be directly installed on the top plate of a bioreactor. Extensive characterization has demonstrated that it exhibits perfect scalability across different sizes, allowing you to initiate perfusion mode right from the start of process optimization and continue it all the way through the production phase.

Applikon BioSep The system has 1 L/ Heaven, 10 L/ Heaven, 50 L/ Heaven, 200 L/ Tianhe 1000 L/ A variety of options, including Tian, are available and can be compatible with bioreactors of any brand.

At cGMP Verified under the specified conditions

Applikon BioSep The system can be used for various industrial operations. cGMP The process's infusion equipment. By default, 50 L/ Tianhe 200 L/ Models with a warranty period of one year or longer include random items. cGMP The verification documents and services required by the standard.

Application

Applikon BioSep Perfectly suited for the following application areas:

Perfused culture
Cell/Particle Concentration
Process Development Research
Cleaning
The first step in downstream processing

BioSep System	1L	10L	50L	200L	1000L
Maximum perfusion rate	1 L/ sky	7 L/ sky	45 L/ sky	200 L/ sky	1000 L/ sky
Minimum perfusion rate	0.1 L/ sky	0.7 L/ sky	4.5 L/ sky	20 L/ sky	100 L/ sky
Separation efficiency	Gundam 99% (Depending on cell concentration and perfusion flow rate)
Minimum cell concentration	2x105 (Depending on cell size)
Bioreactor connection	6 mm outer diameter Pipeline	12 mm outer diameter Pipeline	Install separately	Install separately	Install separately
Culture medium inlet	3 mm Hose barb	6 mm Hose barb	10 mm Hose barb	0.5” TC	0.5” TC
Return pipe connecting to the bioreactor	3 mm Hose barb	6 mm outer diameter Sealing liquid tube	0.5” TC	0.5” TC	0.5” TC
Infusion outlet	3 mm Hose barb	6 mm Hose barb	6 mm Hose barb	0.5” TC	0.5” TC
Weight	0.1 kg	0.5 kg	1.5 kg	13 kg	70 kg
Resonator volume	0.7 ml	7 ml	50 ml	290 ml	1450 ml

BioSep Controller	APS 995	APS 990	APS 990	APS 992	APS 993
BioSep Controller power	15 Wax	150 Wax	150 Wax	350 Wax	1500 Wax
BioSep Controller dimensions (D x W x H, mm)	With my-Control Use in conjunction with	305x130x130	305x130x130	450x400x135	450x300x450
BioSep Controller weight (kg)	With my-Control Use in conjunction with	3.5	3.5	17.6	43