Welcome to Shanghai HanKing Instrument & Equipment Co.,Ltd
Product Category
Interchim puriFlash Multi-Resin Purification
PuriFlash ® 5.250P Purification of Peptide Complex Mixtures
Simple | Efficient PuriFlash 5.250P Specifically designed for the rigorous purification of peptides and oligonucleotides in preparative and rapid chromatography, it consistently separates compounds with very short retention times. UV The flow cell is 1.3mm Reducing the dead volume enables the detection of compounds present in small amounts or with weak absorbance. |
|
|
|
|
|
|
|
|
Intersoft ® X Genius Intelligent software User-friendly interface, easy to operate, and intelligent method development. | Control module 15 Inch touchscreen Remote control USB x 8 RJ45 | Fraction collector 3 a reagent rack 132 pipe 18 x 150 mm Leakage and Waste Disposal System | Chromatographic column Inner diameter can reach 60 mm | Injection system Supports liquid sample injection and dry sample loading. Column balance Load & Go Technology Six-way valve +10 Valve-controlled solid-phase loading | UV :200-400nm UV :200-800nm (Pack-UV extended) Multi-wavelength and full-spectrum scanning for spectral visualization and purity confirmation. Flow cell: 1.3 mm / 55 µL Optional Pack-iELSD |
| Ultraviolet - Visible Spectrophotometer Detector
Choose the UV that suits your needs. - Visible Spectrophotometer Detector
PuriFlash Ultraviolet included with the device - The visible spectrophotometer detector is fully integrated into the acquisition module, and is comprised of... InterSoft X Software control. Since the application is very simple, you only need to enter the desired wavelength into the software and can simultaneously select... 4 a single wavelength, in a manner identical to scanning (the total number of wavelengths available for both models can be included in this model), “Genius” Will be responsible for the acquisition and recording of chromatograms.
Each laboratory has different needs, and since we are eager to meet these needs and always provide you with the right solution, we recommend using two types of UV detectors:
200 – 400 nm (Standard – For proteins PF-020B Except)
200 – 800 nm (Compatible with this option) PuriFlash ® of the UV Expansion pack)
Real-time scanning across the entire wavelength and ultraviolet spectrum
Ultraviolet - The visible spectrophotometer detector is equipped with... “DAD” Technology (diode-array detector). This provides approximately one hundred diodes for scanning the entire wavelength range for detection, enabling real-time acquisition of the UV spectra of eluting compounds.
If you wish, you can at any time—even during operation or reprocessing—return to the peaks in the chromatogram to display the spectrum at the selected time and obtain identification of the elements as well as the purity of the purified compound.
| Integration ELSD Evaporative Light Scattering Detector
Ø ELSD For General detector It can be used very flexibly in either normal-phase or reversed-phase mode, and also supports isocratic separation or multi-solvent gradient methods. ELSD The response factor is always constant.
Ø Compared to a UV detector, ELSD Can detect all compounds. including carbohydrates, peptides, amino acids, fats, polymers, and sugars (polysaccharides, oligosaccharides), PEG And protein.
Ø ELSD With experts in this field To sit Close collaboration aimed at meeting rapid and purification requirements and eliminating common limitations. ELSD Equipped with a fully functional atomizer to eliminate any risk of blockage.
Ø PuriFlash Integrated with ELSD When combined, this method can purify raw materials ranging from a few milligrams to several hundred grams. In routine operations, the maximum elution flow rate (up to... 200mL/min ) and use F0001 To F0800 of the PuriFlash Chromatographic column for high performance.
Ø Low-temperature technology Higher sensitivity is achieved through a unique atomizer and a highly efficient photomultiplier tube.
With this proprietary technology, droplets dry more quickly at low temperatures, providing adequate signal intensity for semivolatile compounds. This technology does not require additional equipment, such as sprayers (spray chambers) with heating systems or evaporation tubes. Peltier Cooling).
Ø Dynamic gain SAGA by Interchim and To sit Co-created, with automatic gain adjustment, enabling... ELSD Without saturation, sensitivity remains unaffected. Detector: High-sensitivity photodiode
Source : LED ( 470nm )
Ambient temperature: up to 100 Celsius
Dynamic: 40 mu L/min sample in DEDL
Gas: 1–1.5 L/min – 1 bar
Ø Through InterSoft X The software performs control.
If not used during the purification process ELSD Then, costs can be saved by turning off the lights and gas.
At the end of each purification, ELSD The dynamic separator automatically cleans itself to ensure proper operation.
|
Configuration |
PuriFlash ® 5.250P |
|
|
Pump |
Flow rate (ml/min) |
125 |
|
Maximum pressure (bar) |
250 |
|
|
Quadruple Gradient Pump |
√ |
|
|
Air venting |
√ |
|
|
Washing discs |
√ |
|
|
Pump Washing Discs (Pack) |
√ |
|
|
Detector |
UV: 200 – 400 nm |
√ |
|
UV: 200 – 800 nm |
Pack-UVextended |
|
|
Purity Confirmation |
√ |
|
|
iELSD (pack) |
pack-iELSD |
|
|
Load sample |
4 Electrically actuated valve |
X |
|
6 Electrically actuated valve w/loop |
X |
|
|
6 Pass-through valve +10 Electrically actuated valve w/loop |
√ |
|
|
Liquid, Dry-Load Load sample |
√ |
|
|
Chromatographic Column Selection Valve |
6 Electrically actuated valve |
Pack-Multi |
|
System Configuration |
Tubings – after pump (ID) |
Peek 1/16” x 0.75mm |
|
Flow cell |
1.3mm/55 mu L |
|
|
Chromatography column holder |
Integration |
√ |
|
Pre-colum Column support |
√ |
|
|
Fraction collection |
Regular |
3 A reagent rack 132 pipe 18x150mm |
|
Control module |
15 Inch touchscreen |
√ |
|
USB |
8 |
|
|
RJ45 |
√ |
|
|
Software |
Intersoft X |
√ |
|
Safety |
Leak detection (pump, collection rack, iELSD , bracket) |
√ |
|
Solvent tray with drainage system |
√ |
|
|
Collector with drainage system |
√ |
|
|
Solvent level monitoring |
√ |
|
|
RFID |
√ |
|
|
Fume Enclosure |
√ |
|
|
Size |
E:D:H (cm) |
40×51×75 |















