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Interchim puriFlash Multi-Resin Purification

Product Details Ordering Information Download Materials Related video Application Cases

 

PuriFlash ® 5.250P

Purification of Peptide Complex Mixtures

 Conventional Flash Purification puriFlash XS 520 Plus Interface Flow rate: 125ml/min   Conventional Flash Purification puriFlash XS 520 Plus Interface Maximum pressure: 250bar

Simple | Efficient

PuriFlash 5.250P Specifically designed for the rigorous purification of peptides and oligonucleotides in preparative and rapid chromatography, it consistently separates compounds with very short retention times. UV The flow cell is 1.3mm Reducing the dead volume enables the detection of compounds present in small amounts or with weak absorbance.

Organic small molecule

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Impurity Identification

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Natural product

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Peptide, oligonucleotide

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Proteins, biopharmaceuticals

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Separation of metabolites

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Trace analysis

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Intersoft ® X Genius Intelligent software

User-friendly interface, easy to operate, and intelligent method development.

Control module

15 Inch touchscreen

Remote control

USB x 8 RJ45

Fraction collector

3 a reagent rack

132 pipe 18 x 150 mm

Leakage and Waste Disposal System

Chromatographic column

Inner diameter can reach 60 mm

Injection system

Supports liquid sample injection and dry sample loading.

Column balance

Load & Go Technology

Six-way valve +10 Valve-controlled solid-phase loading

UV 200-400nm

UV 200-800nm

(Pack-UV extended)

Multi-wavelength and full-spectrum scanning for spectral visualization and purity confirmation.

Flow cell: 1.3 mm / 55 µL

Optional Pack-iELSD

 

| Ultraviolet - Visible Spectrophotometer Detector

 

Choose the UV that suits your needs. - Visible Spectrophotometer Detector

 

PuriFlash Ultraviolet included with the device - The visible spectrophotometer detector is fully integrated into the acquisition module, and is comprised of... InterSoft X Software control. Since the application is very simple, you only need to enter the desired wavelength into the software and can simultaneously select... 4 a single wavelength, in a manner identical to scanning (the total number of wavelengths available for both models can be included in this model), “Genius” Will be responsible for the acquisition and recording of chromatograms.

 

Each laboratory has different needs, and since we are eager to meet these needs and always provide you with the right solution, we recommend using two types of UV detectors:

 

200 – 400 nm (Standard For proteins PF-020B Except)

 

200 – 800 nm (Compatible with this option) PuriFlash ® of the UV Expansion pack)

 

 

Real-time scanning across the entire wavelength and ultraviolet spectrum

 

Ultraviolet - The visible spectrophotometer detector is equipped with... “DAD” Technology (diode-array detector). This provides approximately one hundred diodes for scanning the entire wavelength range for detection, enabling real-time acquisition of the UV spectra of eluting compounds.

 

If you wish, you can at any time—even during operation or reprocessing—return to the peaks in the chromatogram to display the spectrum at the selected time and obtain identification of the elements as well as the purity of the purified compound.

| Integration ELSD Evaporative Light Scattering Detector

Ø   ELSD For General detector It can be used very flexibly in either normal-phase or reversed-phase mode, and also supports isocratic separation or multi-solvent gradient methods. ELSD The response factor is always constant.

 

Ø   Compared to a UV detector, ELSD Can detect all compounds. including carbohydrates, peptides, amino acids, fats, polymers, and sugars (polysaccharides, oligosaccharides), PEG And protein.

 

Ø   ELSD With experts in this field To sit Close collaboration aimed at meeting rapid and purification requirements and eliminating common limitations. ELSD Equipped with a fully functional atomizer to eliminate any risk of blockage.

 

Ø   PuriFlash Integrated with ELSD When combined, this method can purify raw materials ranging from a few milligrams to several hundred grams. In routine operations, the maximum elution flow rate (up to... 200mL/min ) and use F0001 To F0800 of the PuriFlash Chromatographic column for high performance.

 

Ø   Low-temperature technology Higher sensitivity is achieved through a unique atomizer and a highly efficient photomultiplier tube.

 

With this proprietary technology, droplets dry more quickly at low temperatures, providing adequate signal intensity for semivolatile compounds. This technology does not require additional equipment, such as sprayers (spray chambers) with heating systems or evaporation tubes. Peltier Cooling).

 

Ø   Dynamic gain SAGA by Interchim and To sit Co-created, with automatic gain adjustment, enabling... ELSD Without saturation, sensitivity remains unaffected. Detector: High-sensitivity photodiode

 

Source LED ( 470nm )

 

Ambient temperature: up to 100 Celsius

 

Dynamic: 40 mu L/min sample in DEDL

 

Gas: 1–1.5 L/min – 1 bar

 

Ø   Through InterSoft X The software performs control.

 

If not used during the purification process ELSD Then, costs can be saved by turning off the lights and gas.

 

At the end of each purification, ELSD The dynamic separator automatically cleans itself to ensure proper operation.

Configuration

PuriFlash ® 5.250P

Pump

Flow rate (ml/min)

125

Maximum pressure (bar)

250

Quadruple Gradient Pump

Air venting

Washing discs

Pump Washing Discs (Pack)

Detector

UV: 200 – 400 nm

UV: 200 – 800 nm

Pack-UVextended

Purity Confirmation

iELSD (pack)

pack-iELSD

Load sample

4 Electrically actuated valve

X

6 Electrically actuated valve w/loop

X

6 Pass-through valve +10 Electrically actuated valve w/loop

Liquid, Dry-Load Load sample

Chromatographic Column Selection Valve

6 Electrically actuated valve

Pack-Multi

System Configuration

Tubings – after pump (ID)

Peek 1/16” x 0.75mm

Flow cell

1.3mm/55 mu L

Chromatography column holder

Integration

Pre-colum Column support

Fraction collection

Regular

3 A reagent rack

132 pipe 18x150mm

Control module

15 Inch touchscreen

USB

8

RJ45

Software

Intersoft X

Safety

Leak detection (pump, collection rack, iELSD , bracket)

Solvent tray with drainage system

Collector with drainage system

Solvent level monitoring

RFID

Fume Enclosure

Size

E:D:H (cm)

40×51×75

Introduction to AIS Purification and Preparation Systems and Organic Mass Spectrometry Products

5.2MB

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