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2026

Analysis of Chuanxiong (Ferulic acid, Ligustilide): Refer to the Chinese Pharmacopoeia (2025 edition)

According to the analysis method for measuring Chuanxiong content in the 2025 edition of the Chinese Pharmacopoeia, using CAPCELL PAK C18 MGII and DAISOPAK SP-ODS-RPS columns, you can get good analytical results for both ferulic acid standards and Chuanxiong samples, as well as for dibenzoylmethane standards and Chuanxiong samples.

According to the analysis method for measuring Chuanxiong content in the 'Chinese Pharmacopoeia' (2025 edition), using CAPCELL PAK C18 MGII and DAISOPAK SP-ODS-RPS columns, you can get good analytical results for ferulic acid standards and Chuanxiong samples, as well as for dl-menthol standards and Chuanxiong samples.

Determination of Ferulic Acid Content in Chuanxiong

According to the analysis method for ferulic acid content in Chuanxiong in the 25th edition of the Chinese Pharmacopoeia, we used two mid-grade CAPCELL PAK C18 MGII and DAISOPAK SP-120-5-ODS-RPS columns to analyze the ferulic acid standard solution and Chuanxiong sample solution. The results are shown in Figures 1 and 2 and Tables 1 and 2. Both columns provided good analysis results for the ferulic acid standard solution and the Chuanxiong sample solution.

The CP C18 MGII column analysis of ferulic acid standard shows a theoretical plate number of 17,499, and the DP SP-ODS-RPS column analysis shows a theoretical plate number of 16,479. The theoretical plate numbers for the ferulic acid peaks are all higher than the Pharmacopoeia requirement of 4,000.

图1.png

Figure 1 CP C18 MGII Analysis Spectrum

Table 1 Results of Peak Integration

表1.png

图2.png

Figure 2 DP SP-ODS-RPS Analysis Spectra

Table 2 Results of Peak Integration

表2.png

【Chromatography condition】

Column：CP C18 MGII S5; 4.6×250mm

DP SP-ODS-RPS S5; 4.6×250mm

Mobile phase: methanol / 1% acetic acid solution = 30 / 70

flow rate：1.0mL/min

Column Temperature：35℃

Detector：UV 321nm

Injection volumn：10 μL

Reference standard：10mg/mL，Prepared according to pharmacopoeia methods

Reference standard：20μg/mL

Solvent: 70% methanol

Preparation of Test Sample: Take about 0.5g of the powder of this product (sieved through a No. 4 sieve), accurately weigh it, place it in a stoppered conical flask, add 50 mL of 70% methanol precisely, seal tightly, weigh, heat and reflux for 30 minutes, let it cool, then weigh again, add 70% methanol to make up for any loss in weight, shake well, let it sit, take the supernatant, filter it, and collect the filtrate to obtain the sample.

Determination of Ligustilide Content in Chuanxiong

According to the analysis method for determining the content of senkyunolide in Chuanxiong in the 25th edition of the Chinese Pharmacopoeia, using a DAISOPAK SP-120-5-ODS-RPS column, the control solution of butylphthalide and the Chuanxiong test solution were analyzed. This gave good analytical results with baseline separation of all impurities in the Chuanxiong test solution. The retention time of butylphthalide was 29.169 min, the retention time of senkyunolide was 48.05 min, and the relative retention time to the butylphthalide peak was 1.647.

The analysis results of the DP SP-ODS-RPS column are shown in Figures 3 and 4 and Table 3. In the sample analysis, each peak achieved baseline separation with a resolution greater than 1.5. For the standard analysis, the theoretical plate number of the dibenzoyl peak was 20,314, meeting the pharmacopeia requirement of greater than 3,000.

图3.png

图3 DP C18 RPS分析图-230 nm

图4.png

Figure 4 DP SP-ODS-RPS Analysis Chart - 330 nm

Table 3 Peak Integration Results

表3.png

【Chromatography Conditions】

Column：DP SP-ODS-RPS S5; 4.6×250 mm

Mobile phase: methanol / water = 52 / 48

Flow rate：1.0 mL/min

Column temperate：35 ℃

Detector：Dibenzoylmethane UV 230 nm; Goshonoside UV 330 nm

Injection volumn：5 μL

Test item：8 mg/mL，Prepared according to the pharmacopeia method

Reference product：80 μg/mL

Reference product:

Sample preparation: Take about 0.2 g of the powdered sample (sieved through a No. 4 sieve), weigh it accurately, place it in a stoppered conical flask, add 25 mL of methanol precisely, seal it tightly, weigh it, sonicate for 30 minutes, let it cool, weigh again, make up for any loss with methanol, mix well, filter, collect the filtrate, and store it in a brown bottle—it's ready to use.

In summary, according to the analysis method for determining the content of Chuanxiong in the 25th edition of the 'Chinese Pharmacopoeia', among them

1. For ferulic acid, using either the CAPCELL PAK C18 MGII or DAISOPAK SP-120-5-ODS-RPS columns can give good analysis results for both ferulic acid standards and Chuanxiong samples.

2. Using the DAISOPAK SP-120-5-ODS-RPS column under γ-butyrolactone, you can get good analysis results for both the phthalonitrile standard and Chuanxiong samples.

Keywords:

ASAP-CMS Direct Sampling: Opening the 'Shortcut' to Mass Spectrometry Analysis of Polymer Conductive Materials

Analysis of Inosine, referring to the Chinese Pharmacopoeia (2025 edition), Part II