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12
2026
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08
Analysis method of Copper Tripeptide-1 according to 'YY/T 10005-2026 Raw Materials for Cosmetics - Copper Tripeptide-1'
In this experiment, according to the methods in the standards, we used a DAISOPAK ODS-P regular C18 column and a CAPCELL PAK ADME-HR strong polar column to measure tripeptide-1 copper. The specific experimental results are as follows.
Copper tripeptide-1 has become a star ingredient in the cosmetics world in recent years. It can boost the production of collagen (types I, III, IV) and elastin, reduce fine lines and wrinkles, improve skin elasticity, increase the production of intercellular substances like ceramides, help repair skin damage, provide antioxidant benefits, and brighten the complexion. It's multi-functional and gentle.
Copper Tripeptide-1
C14H22CuN6O4 401.91
On April 3, 2026, the National Medical Products Administration released the cosmetic industry standard "YY/T 10005-2026 Raw Materials for Cosmetics - Copper Tripeptide-1," which will take effect on May 1, 2027.
In this experiment, according to the methods in the standard, Copper Tripeptide-1 was tested using DAISOPAK ODS-P regular C18 columns and CAPCELL PAK ADME-HR strongly polar columns. The specific experimental results are as follows.
01
ODS-P Column
According to the standard method, DAISOPAK SP-100-5-ODS-P S5; 4.6 mm i.d. × 250 mm column was used to analyze the copper-tripeptide-1 raw material. The chromatogram is shown in Figure 1, and the integration table is shown in Table 1.
Copper ions and tripeptide-1 are well separated, with no obvious interfering peaks. The theoretical plate number calculated based on the tripeptide-1 peak is 10,232, which is above the minimum of 2,500; calculated based on the copper ion peak, it is 9,502, above the minimum of 3,000, both meeting the standard requirements.
Figure 1 DP ODS-P Analysis Results
Table 1 Points Table
【Chromatography conditions】
Column：DP ODS-P S5; 4.6×250 mm
Mobile phase：A，Aqueous solution containing 0.1% trifluoroacetic acid;
B，Acetonitrile solution containing 0.1% trifluoroacetic acid;
flow rate：1.0 mL/min
Column temperature：30 ℃
Check：PDA 220nm
- Injection volumn：10μL
Preparation of the test solution: Accurately weigh 50 mg of the test substance (accurate to 0.1 mg), place it in a 25 mL volumetric flask, add blank solution (0.1% trifluoroacetic acid in water) to dissolve it, use ultrasound to help dissolve, then dilute to the mark and mix well as the test substance stock solution. Accurately measure 1.0 mL of the stock solution, put it in a 50 mL volumetric flask, add blank solution to dilute to the mark, and mix well. Filter through a 0.45 µm microporous filter membrane, and use the filtrate as the test solution.
02
ADME-HR Column
Using the CAPCELL PAK ADME-HR S5; 4.6mm i.d. × 250mm column, the tripeptide-1 copper raw material was analyzed, and the chromatogram was obtained as shown in Figure 2, with the integration table shown in Table 2.
Copper ions and Tripeptide-1 are well separated with no obvious interference peaks. The theoretical plate number calculated for the Tripeptide-1 peak is 8,903, which is above 2,500; for the copper ion peak, it’s 14,254, which is above 3,000. Both meet the standard requirements.
Figure 2 ADME-HR Analysis Results
Table 2 Points Table
【Chromatography conditions】
Column：CP ADME-HR S5; 4.6×250 mm
Mobile phase：A，含0.1 % Aqueous solution of trifluoroacetic acid;
B，Acetonitrile solution containing 0.1% trifluoroacetic acid；
flow rate：1.0mL/min
Column Temperature：30 ℃
Check：PDA 220nm
Injection column：10μL
Preparation of the test solution: Accurately weigh 50 mg of the test substance (accurate to 0.1 mg), place it in a 25 mL volumetric flask, add blank solution (0.1% trifluoroacetic acid in water) to dissolve it, use ultrasound to help dissolve, then dilute to the mark and mix well as the test substance stock solution. Accurately measure 1.0 mL of the stock solution, put it in a 50 mL volumetric flask, add blank solution to dilute to the mark, and mix well. Filter through a 0.45 µm microporous filter membrane, and use the filtrate as the test solution.
In summary, using either the DAISOPAK ODS-P S5; 4.6×250 mm column from DAISO Sanya or the CAPCELL PAK ADME-HR S5; 4.6×250 mm column, and following the method in 'YY/T 10005-2026 Cosmetics Raw Materials Tripeptide-1 Copper', it's possible to achieve good detection of copper ions and Tripeptide-1, with results meeting the method's requirements.
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