REBEL: A biochemical analyzer capable of rapidly analyzing the amino acid and vitamin composition of cell culture media.

REBEL is a highly integrated analyzer that, with its small sample volume and rapid analysis time, helps accelerate the optimization of process development strategies.

REBEL: A biochemical analyzer capable of rapidly analyzing the amino acid and vitamin composition of cell culture media.

2023-11-21

In process development and production, it is essential to monitor key nutrient components of the culture medium and cellular metabolites in bioreactors or other cell-culture tools at several critical time points. REBEL It is a highly integrated analyzer that can... Small sample size, rapid analysis time , helping to accelerate the optimization of process development strategies. REBEL Simply centrifuge or filter the sample and then dilute it—this is faster than traditional methods. LC or LC-MS The method is much simpler and the analysis time is shorter.

One, CHO Cell culture medium analysis

CHO Cell CDM Culture media typically come in two forms: liquid and dry powder. When deciding which type of culture medium to use, researchers must first determine the quantity of medium required for their project. Liquid media can be directly used in small-scale bioreactors, shake flasks, and microplates. In contrast, dry powder media must be reconstituted before use; however, dry powder media are generally easier and cheaper to transport and store, making them more commonly used for larger-scale cultures. Many companies have dedicated media preparation personnel and laboratories specifically tasked with reconstituting dry powder media, thereby streamlining workflows for cell culture and upstream process development. Rapidly analyzing the composition of fresh media prior to supplementation can ensure consistency in medium composition both before and after scale-up or reconstitution.

The concentrations reported above are derived from dry powders and liquids. CDM Analysis of the culture medium formulations (neither of the two medium mixtures contains glutamine). For the dry powder, prepare according to the manufacturer’s instructions. All samples were used... REBEL Diluent dilution 10 Twice, the reported concentration is 5 The average of the second replicate test.

II. Analysis of Culture Media Containing Serum and Other Additives

CDM The compositional analysis of the culture medium may be affected by serum ( FBS ) and becomes more complex due to the presence of other additives. In the figure, CDM The concentration of components in the culture medium is not affected by the presence of fetal bovine serum. 0-25% , as shown on the left in the figure). In the second experiment (shown on the right in the figure), CDM The reported component concentrations in the culture medium were also unaffected by several other different additives. Such results are significant for... REBEL As for this, simply centrifuge or filter the sample and then dilute it with a diluent. 100 Twice the amount—no additional sample preparation is required.

Three, T Cell culture medium analysis

With the introduction of more animal-free, commercially available media, understanding... T It is important to supplement specific components during cell culture, as this can more easily ensure consistency in the production process. Adopting... REBEL Detection RPMI + Serum, with or without added serum combo ( Basic and Cell Expansion Version ) The essential amino acid content of the basal medium alone and the cell expansion medium alone.

Four, HEK293 CDM Culture Medium Analysis

From four suppliers HEK293 The culture media were tested to identify differences among various media formulations for the same type of cells. Before analysis, the culture media were diluted. 100 Twice ( n = 5 ). Overall, from the supplier 3 The concentrations of several components in the culture medium are the highest.

V. Analysis of Mesenchymal Stem Cell Culture Medium

Two different mesenchymal stem cell culture media and supplements were tested. The culture media were diluted prior to analysis. 10 Twice ( n = 5 ). When adding a replenishment solution, the culture medium 1 There was no difference in amino acid levels, but in the culture medium... 2 middle, Gly 、 Island 、 Party 、 Pro 、 She is 、 Thr, 、 Trp and Val The concentration has increased significantly.

Usage and Sample Handling:

What you see is what you get.

REBEL The side does not require an external computer, compressed gas cylinders, waste containers, or reagent bottles.

All you need to do is pick the item. (1) Centrifuge or filter afterward to remove cells, and then use... REBEL Diluent (2) Dilute. Then load the sample into the device. (3) , according to “start” (4) All calibration standards and reagents are stored in... REBEL Inside the instrument, the data will be automatically processed into a report. CSV or PDF ), available You Disk export (5) 。

Researchers not only need to ensure that the cell culture medium they are using is suitable for their application, but also should regularly analyze changes in the concentrations of its components during transportation, storage, and cultivation to make sure that any variations in cell growth and production are not attributable to changes in the cell culture medium formulation. / arising from unknown variations in ingredient concentrations. And REBEL It will be a powerful assistant for doing these things.

Keywords:

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