[Technical Sharing] Thin-layer chromatography-mass spectrometry coupling—get mass spectrometry information in just 30 seconds!

[Technical Sharing] Thin-layer chromatography-mass spectrometry coupling—get mass spectrometry information in just 30 seconds!

2023-08-29

color Spectral methods originated from 20 At the beginning of the century, 1906 In the year, Russian botanist Mikhail Tsvet filled a glass tube with calcium carbonate and used petroleum ether to elute extracts of plant pigments. After a period of elution, the plant pigments were separated within the calcium carbonate column, transforming a single band into several parallel bands. Building on the principles of chromatography, scientists later developed thin-layer chromatography. Thin-layer chromatography ( TLC It has the advantage of rapidly separating complex mixtures and serves as a highly useful tool for monitoring reactions; it can also be applied to the selection of solvents in column chromatography separations. This method is now widely used in chemical laboratories, for example in areas such as organic synthesis and natural product analysis.

Advion Use thin-layer chromatography - Mass spectrometry coupled method

30s You can obtain mass spectrometry information.

Traditional TLC Method VS TLC-CMS Method

Traditional TLC For spot analysis of plate samples, it is necessary to scrape off the target spot, dissolve it in a suitable solvent, centrifuge to separate the supernatant, and then dry it under air before dissolving it in a solvent suitable for GC-MS or LC-MS analysis. The entire process can take anywhere from a few hours to several days, is time-consuming and labor-intensive, and its efficiency cannot be guaranteed!

2015 Year, Advion Independently developed and launched Plate Express Thin-layer chromatography-mass spectrometry interface , can quickly from TLC Samples are extracted from the plate and transmitted online to mass spectrometry for detection, compared to the traditional... TLC Method, TLC-CMS More convenient and reliable, 30 Obtaining sample mass spectrometry information in seconds greatly enhances research efficiency and truly enables rapid, in-situ detection!

TLC-CMS Thin-layer chromatography - Mass spectrometry coupled with...

Eluting solvent → TLC Extract → Mass spectrometry identification → Data analysis, 30S Obtain mass spectrometry information internally!

TLC-CMS The experimental method takes three steps.

Step 1 : Setup method

Step 2 ： Place the thin-layer chromatography plate and click. “ Run ”

Step 3 ： Directly read sample mass spectrometry information

TLC Advantages of the Thin-Layer Chromatography-Mass Spectrometry Interface

1. Software-based automated control with simple operation steps;

2. After separation by thin-layer chromatography, the sample requires no further processing and can be directly sampled for mass spectrometry analysis, ensuring single-point elution.

3. Flexibly set the extraction intensity and duration for samples, optimizing extraction based on different thin-layer plates; compatible with most commercially available thin-layer plates.

4. Replaceable sealing elution heads of different thicknesses;

5. To prevent cross-contamination, the elution tip is automatically cleaned after each analysis.

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