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2024
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Maverick Spectral Raw Data Analysis and OPC UA Communication
MAVERICK Spectral raw data analysis and O PC UA Communication
Maverick Device Overview
The MAVERICK, a product of 908 Devices, is an online process analytical technology (PAT) device based on Raman spectroscopy. It... Use Raman spectroscopy technology, built on the chemistry of media and device physics rather than empirical data. De Novo Model ， It can automatically interpret spectra and quantify process parameters, thereby enabling the measurement of glucose, lactic acid, and... Total cell density ( TCD) Simultaneous measurement 。
Although of 908 Devices' MAVERICK device Brand new Modeling in Biological Process Analysis It provides a plug-and-play, low-cost, and rapid solution. However, we must acknowledge that the parameters it currently offers are still limited. This may constrain its use in certain specific application scenarios. The scope of application and effectiveness of the MAVERICK device.
However, it is worth noting that, MAVERICK device It is also an excellent Raman spectroscopy detection device. To address this limitation, The MAVERICK device also provides raw data. Output This means that users can Using the raw spectral data, Based on your own needs and goals, analyze this data. Modeling And process ， This provides users with greater flexibility. 。
MAVERICK data format
The MAVERICK device saves raw spectral data in HDF5 format, with the file extension “.MSP” (MAVERICK Spectral file). HDF5 is an open-source, non-profit, managed format originally designed to support large datasets in cloud and parallel computing environments. Most modern modeling and computational software platforms support the HDF5 file format, including Python, Matlab, SAS, JMP, and R. Although SIMCA Not natively supported HDF5 File, but it can be used. Python Script (Provide sample code) To put MAVERICK Data loaded to SIMCA In a compatible project format.
MAVERICK Acquisition Method 
During measurement operation, MAVERICK continuously and automatically acquires alternating pairs of bright-field and dark-field images from the spectrometer’s CCD. Both bright-field and dark-field images are saved in MSP files. MAVERICK’s exposure time per image frame is 5 seconds. Each bright-field image is acquired over a 5-second period under laser illumination, while each dark-field image is acquired for an equivalent duration but without laser illumination. MAVERICK will continue to acquire and save these bright-field and dark-field image pairs until... Measurement Suspended or stopped.
These data will all be retained. M SP In the document.
MAVERICK Post-processing Method
Recommended post-processing methods Including dark-field subtraction and response. /Quantum efficiency correction. Dark-field subtraction involves subtracting the dark-field image from the bright-field image, which can eliminate artifacts in measurements such as detector dark current, ADC voltage offsets, and stray light. Response/quantum efficiency correction is strongly recommended when using or comparing data from multiple MAVERICK devices (or other Raman spectrometers).
Maverick’s Special Features and Precautions
When to... When comparing Maverick’s Raman spectral data with data acquired by other Raman systems—especially in terms of signal-to-noise ratio—it is essential to take into account the differences in exposure time and laser power. Due to MAVERICK’s acquisition time is typically much faster than that of other systems, which is crucial for real-time control loops and ambient light management. ， Therefore, one should not simply... The 5-second spectrum from MAVERICK is compared with the 30-minute spectrum from other systems. In post-processing, the average can be increased as needed to match the desired equivalent exposure time. For example, averaging six processed Raman spectra is equivalent to a collection time of 1 minute, while averaging 60 processed Raman spectra is equivalent to a collection time of 10 minutes. Time.
MAVERICK Raman spectral data compared with other commercially available ones on the market. 785 nm The stimulated Raman platform is very similar. Some noteworthy aspects include:
- Useful Raman Displacement The range is approximately 300 To 3200 cm⁻¹ (Although MSP The document provides less than 300 cm⁻¹ the data)
- Liquid / The Raman characteristics in the solid phase have approximately 6-8 cm⁻¹ the inherent linewidth. Spectrometers with resolutions lower than this linewidth show no significant effect on the linewidth. Effect 。
- Obtained from biological processes MAVERICK The data will show characteristics such as the Raman-active components of water and the sample.
- Several Raman peaks will appear in the sapphire. Displacement With. Their existence and intensity are intentional. righteousness of the ——MAVERICK The continuous performance validation checks rely on these. Displacement With. MAVERICK These sapphires are also used in the brand-new model. Displacement Feature.
- If MAVERICK Several key performance-validation features—such as sapphire, water, and several other proprietary aspects—cannot be observed in Raman spectra, and in fact, they can disrupt data acquisition. Therefore, it cannot be used as a general-purpose Raman spectrometer.
MAVERICK's OPC UA features
Maverick’s strengths lie not only in its powerful Raman data processing capabilities but also in its unique expertise in industrial communication standards. It can not only output analog signals, but also... Compared to other competitors in the market, A major advantage of MAVERICK is its comprehensive support for OPC UA (OLE for Process Control Unified Architecture).
OPC UA is a data exchange standard used for industrial automation and communication. It originated in 1994 and was developed by the OPC Foundation, a group of software and hardware vendors in the industrial automation sector. The advantages of the OPC UA protocol include its flexibility in cross-platform operation, data security, and scalability as data is transformed into information. In the biopharmaceutical field, OPC UA provides the foundational building blocks for constructing control system workflows.
How to Use OPC UA in MAVERICK
1. Connect O PC UA Client
To use With MAVERICK’s OPC UA functionality, all you need to do is connect the MAVERICK Hub to your local network. Immediately At System Information Entry In the middle, find the one assigned to MAVERICK's IP address .(Image 1) 
Figure 1. The system information screen displays MAVERICK IP. Address
To read data, You Need OPC-UA client. We take “UaExpert Client” (As shown in the figure) 2) as an example, This software can be used free of charge. No matter which client you use, its The data structures are all identical because the structure is determined by... Created by the OPC-UA server, but the client program may differ in how it presents and arranges the menu.
Figure 1. The system information screen displays MAVERICK IP. Address
To read data, You Need OPC-UA client. We take “UaExpert Client” (As shown in the figure) 2) as an example, This software can be used free of charge. No matter which client you use, its The data structures are all identical because the structure is determined by... Created by the OPC-UA server, but the client program may differ in how it presents and arranges the menu.
Figure 2. UaExpert and version information
To add OPC server, please select “Server” ‒ > Add or select the “+” symbol (the orange circle in Figure 3).
Figure 3. Add server menu and toolbar buttons
Next, double-click. Under “Custom Discovery,” click “Double-click to Add Server...,” then enter the IP address of MAVERICK, as shown in Figure 4.
Figure 4. Enter the server's URL
After entering this information, you will see this window, in which... “ O The “K” button is gray. (For example, Figure 5)
Figure 5. Add server - Self-defined discovery
Next, click the arrow next to the magnifying glass symbol, then double-click. The “NodeOPCU” text displays the “Replace Hostname” window. ， Press “ Yes Continue .(Image 6)
Figure 6. Replace the hostname
This will produce List of encryption options under the OPC UA server. Select “ None ”, set the identity verification option to “Anonymity”. Click “OK”. (Figure 7)
Figure 6. Replace the hostname
This will produce List of encryption options under the OPC UA server. Select “ None ”, set the identity verification option to “Anonymity”. Click “OK”. (Figure 7)
Figure 8. Connect to the server
When you connect to the server for the first time, you may encounter this certificate verification window warning indicating that the certificate is not trusted. Select... “Trust Server Certificate.” This should cause the status to change from “Bad Certificate Untrusted” to “Good.” Select. Continue ”。 (Figure 9)
Figure 9. Certificate verification
II. Data Structures
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Data structures are composed of OPC-UA The server transmits and displays on “Address Space” Middle. (Image 10 )
Figure 10. Data access view Figure
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You can also right-click anywhere in the white area of the data access view and select. “add custom node” Add the analyte to the data access view. Add Node ID Paste into the text box, and the first input box. “NsIndex” Set to 1 Please note that the value in the first input box should always be 1. Figure 13 Shows that all have been added. MAVERICK Data access view of the node.
Figure 13. All Mavericks displayed in the data access view Festival Point
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